Some subdivisions extend through more than one region and division. Only the portion of the subdivision in this division is shown.
| Subdivision | From | To | Length | Spurs | Authority | Time Table |
|---|---|---|---|---|---|---|
| Stamford | Black Rock, Ontario | Clifton, Ontario | 31.6 miles | 11 | TT&TO & CTC | Stamford |
| Grimsby | Suspension Bridge, Ontario | Hamilton, Ontario | 43.7 miles | 8 | CTC & ABS | Grimsby |
| Dunnville | Fort Erie Yard, Ontario | Simpson, Ontario | 75.4 miles | 3 | MBS | Dunnville |
| Humberstone | Nickel, Ontario | Yager, Ontario | 5.9 miles | 1 | CTC | Humberstone |
| Thurold | Port Robinson, Ontario | Merritton, Ontario | 7.9 miles | 4 | MBS & CTC | Thurold |
| Canal | Feeder West, Ontario | Thurold, Ontario | 14.2 | 4 | CTC & ABS | Canal |
| Cayuga | Robbins, Ontario | Feeder West, Ontario | 20.9 miles | 2 | CTC | Cayuga |
| Hagersville | Hamilton, Ontario | Nanticoke, Ontario | 35.7 miles | 7 | MBS | Hagersville |
| Oakville | Long Branch, Ontario | Hamilton, Ontario | 29.9 miles | 24 | CTC 2 & & tracks | Oakville |
| Burford | Brant Junction, Ontario | end of steel | 33.4 miles | 1 | MBS | Burford |
| Halton | Tansley, Ontario | Burlington West, Ontario | 6.4 miles | 1 | CTC 2 tracks | Halton |
| Drumbo | Paris Junction, Ontario | Bright, Ontario | 14 miles | 0 | TT&TO | Drumbo |
| Dundas | Bayview, Ontario | Paris Junction, Ontario | 33.5 miles | 4 | CTC | Dundas |